PHYB / Anti-Phytochrome B antibody Product Information   Description: Mouse monoclonal (Clone: 10P5) antibody   Background: PHYB is a red/far-red photoreceptor involved in the regulation of de-etiolation. It exists in two inter-convertible forms: Pr and Pfr (active). It is involved in the light-promotion of seed germination and in the shade *oidance response.   Synonyms: PHYB, HY3, OOP1, OUT OF PHASE 1, PHYTOCHROME B   Immunogen: Recombinant protein of PHYB derived from Arabidopsis thaliana AT2G18790.   Form: Lyophilized   Quantity: 150 g   Purification: Protein A purified   Reconstitution:   Reconstitution with 150l of 0.01M sterile PBS.  "Note: please spin tube briefly prior to opening it to *oid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".   Stability &        Storage: Use a manual defrost freezer and *oid repeated freeze-thaw cycles.  12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution.  1 month, 2 to 8℃ under sterile conditions after reconstitution.   Shipping: The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. Application Information   Applications:  Western Blot (1:1000-1:2000)  Note: Optimal dilutions/conce*ations should be determined by the end user.   Expected Results:  125 kDa   Confirmed Reactivity:  Arabidopsis thaliana   Predicted Reactivity:  For more species homologues information, please contact tech support at tech@phytoab.com. Application Example Lane 1: 35 μg total protein from phyB-9 mutant of Arabidopsis thaliana leaf. Lane 2: 35 μg total protein from Arabidopsis thaliana leaf.  Electrophoresis: 8% SDS-Urea-PAGE Transfer: blotting to NC (nitrocellulose) membrane for 1h. Blocking: 5% skim milk at RT or 4℃ for 1h. Primary antibody: 1:1000 dilution overnight at 4℃. Secondary antibody: 1:5000 dilution using Goat Anti-Mouse IgG H&L (HRP). Detection: using chemiluminescence substrate and image were captured with CCD camera. 标签： 光敏色素   光敏色素   北京市phyb光敏色素   北京市phyb光敏色素厂家
[光敏色素] 北京市phyb光敏色素厂家
PHYB/光敏色素

本文地址：https://www.zhuigan.cn/product/853632.html